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The CellStore Eden Rocking Bioreactor System is a single-use, automated platform for scaled CGT cell culture—delivering a scalable, automated, and stable culture environment for gene and cell therapy

Eden Rocking Bioreactor System

The CellStore Eden Rocking Bioreactor System is a single-use, automated platform for scaled CGT cell culture—delivering a scalable, automated, and stable culture environment for gene and cell therapy


Product Advantages


Flexible Culture Capacity

Interchangeable 10 L / 20 L / 50 L trays, compatible with single-use cell culture bags in 2 L, 10 L, 20 L, and 50 L formats. Working volumes from 0.5 L to 25 L—one system covers your needs from process development through pilot and production scale.


Proven, Ready-to-Deploy Processes

Established, robust fed-batch and perfusion processes for immune cells and HEK293 cells enable rapid process implementation and transfer.


Precise Process Control 

Real-time monitoring of temperature, pH, DO, gas supply, and feeding, with automated control throughout the culture run.


Standardized Process Management 

A high-precision control system continuously monitors and adjusts critical process parameters, with data acquisition, real-time trending, historical record tracing, batch-to-batch comparison, and report printing—designed to meet GMP requirements.


Single-Use Consumables — Single-Use Bags That Simplify Your Workflow

Single-use cell culture bags and media storage bags for suspension cell culture and scale-up processes, supported by comprehensive consumables validation documentation.

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Automated Operation — Automated Control, Minimized Manual Intervention

Cascade control of weight, peristaltic pumps, pH, and CO₂ executes the culture process automatically according to pre-set recipes—reducing manual intervention and run-to-run variability. 


Performance Data


Large-Scale NK Cell Expansion Case Study

Frozen CBMC  |  Initial seeding: 5.2 × 10⁷ cells  |  CellStore Eden 10 L  |  20-day culture

≈ 6.0 × 10¹⁰≈ 900×≥ 95%99%
Total NK cells harvestedExpansion foldCell viability throughoutCD3⁻CD56⁺ NK cell purity



       

cellviability-curve-02.png

Data generated using the CellStore Eden 10 L rocking bioreactor with the CellStore NK cell culture system.


Technical Specifications


Tray10 L / 20 L / 50 L
Insulated tray cover10 L / 20 L / 50 L
Power supplyAC 220 V, 50/60 Hz, 6.9 A
Power consumption1500 VA
System Base
Rocking speed control range1–40 rpm
Rocking angle control range1°–12°
Rocking acceleration range15%–100%
Culture media weight control range0.5–25 kg
Temperature control range(Ambient + 5 °C) to 40 °C ¹
Temperature control accuracy±0.2 °C
Gas Control
Gas flow rate range ²0.025–0.5 L/min
CO₂ control range0–20% CO₂
O₂ control range0–50% O₂ (when blended with N₂); 21–50% O₂ (when blended with air)
Exhaust gas controlExhaust condenser; heated exhaust filter assembly
PH & DO Control
PH measurement range (single-use optical sensor)pH 5.5–8.5
PH control rangepH 6.0–8.0
PH control accuracy±0.05 pH
DO measurement range (single-use optical sensor)0–200% air saturation
DO control accuracy±5% air saturation (excluding barometric variation)


Notes:  1. Optional temperature control range: ambient + 5 °C to 60 °C.  2. Optional 1, 3, or 4 thermal mass flow meters; flow meter ranges customizable on request.  3. Optional 2 variable-speed peristaltic pumps (standard pump heads: small 0.1–36 L/D, large 0.1–260 L/D); pump speed customizable on request. Pump flow accuracy after calibration: ±(0.1% of reading + 5%) mL/min. Optional external peristaltic pumps and external weighing system available. Specifications are subject to change without notice.


Single-Use Cell Culture Bags


Suspension cell culture bags enable direct suspension culture of cells. The cell culture bag with cell retention membrane retains cells inside the bag through a membrane of defined pore size, enabling high-density suspension cell culture and perfusion culture.

Total volume (L)2 / 5 / 10 / 20 / 50
Working volume (L)0.5–25
ConnectorsMale / female Luer connectors; quick-connect fittings
FiltersInlet air filter, exhaust filter
PortsSampling, feed, harvest, perfusion inlet, and outlet ports
SensorspH optical sensor / DO optical sensor
Perfusion modeBuilt-in retention membrane


Ordering Information


Product

Cat. No.

Description

Eden Rocking Bioreactor

CS-Eden-01

Bioreactor Main Unit — Configured for control of speed, angle, temperature, pH/DO, gas flow rate, CO₂ concentration, perfusion/weight control, and pressure. Configuration includes 1 control cabinet with a color touchscreen display and 1 rocking platform main unit. Tray protective cover not included.

10 L Insulated Tray Cover

CS-TC-010

10 L Tray with Insulated Cover

20 L Insulated Tray Cover

CS-TC-020

20 L Tray with Insulated Cover

50 L Insulated Tray Cover

CS-TC-050

50 L Tray with Insulated Cover

2 L Cell Culture Bag with Cell Retention Membrane

CS-CBRM-002

Total Volume: 2 L; Working Volume: 0.5–1 L

5 L Cell Culture Bag with Cell Retention Membrane

CS-CBRM-005

Total Volume: 5 L; Working Volume: 0.5–2.5 L

10 L Cell Culture Bag with Cell Retention Membrane

CS-CBRM-010

Total Volume: 10 L; Working Volume: 1–5 L

20 L Cell Culture Bag with Cell Retention Membrane

CS-CBRM-020

Total Volume: 20 L; Working Volume: 2–10 L

50 L Cell Culture Bag with Cell Retention Membrane

CS-CBRM-050

Total Volume: 50 L; Working Volume: 5–25 L

2 L Cell Culture Bag with Cell Retention Membrane (without pH/DO sensors)

CS-CBRMN-002

Without pH and DO sensors; Total Volume: 2 L; Working Volume: 0.5–1 L

5 L Cell Culture Bag with Cell Retention Membrane (without pH/DO sensors)

CS-CBRMN-005

Without pH and DO sensors; Total Volume: 5 L; Working Volume: 0.5–2.5 L

10 L Cell Culture Bag with Cell Retention Membrane (without pH/DO sensors)

CS-CBRMN-010

Without pH and DO sensors; Total Volume: 10 L; Working Volume: 1–5 L

20 L Cell Culture Bag with Cell Retention Membrane (without pH/DO sensors)

CS-CBRMN-020

Without pH and DO sensors; Total Volume: 20 L; Working Volume: 2–10 L

50 L Cell Culture Bag with Cell Retention Membrane (without pH/DO sensors)

CS-CBRMN-050

Without pH and DO sensors; Total Volume: 50 L; Working Volume: 5–25 L

2 L Suspension Cell Culture Bag (with pH/DO sensors)

CS-CBS-002

With pH and DO sensors; Total Volume: 2 L; Working Volume: 0.5–1 L

5 L Suspension Cell Culture Bag (with pH/DO sensors)

CS-CBS-005

With pH and DO sensors; Total Volume: 5 L; Working Volume: 0.5–2.5 L

10 L Suspension Cell Culture Bag (with pH/DO sensors)

CS-CBS-010

With pH and DO sensors; Total Volume: 10 L; Working Volume: 1–5 L

20 L Suspension Cell Culture Bag (with pH/DO sensors)

CS-CBS-020

With pH and DO sensors; Total Volume: 20 L; Working Volume: 2–10 L

50 L Suspension Cell Culture Bag (with pH/DO sensors)

CS-CBS-050

With pH and DO sensors; Total Volume: 50 L; Working Volume: 5–25 L

500 mL Suspension Cell Culture Bag (without pH/DO sensors or retention membrane)

CS-CBSN-0005

Total Volume: 500 mL; Working Volume: 50–250 mL

2 L Suspension Cell Culture Bag (without pH/DO sensors or retention membrane)

CS-CBSN-002

Without pH and DO sensors; Total Volume: 2 L; Working Volume: 0.5–1 L

5 L Suspension Cell Culture Bag (without pH/DO sensors or retention membrane)

CS-CBSN-005

Without pH and DO sensors; Total Volume: 5 L; Working Volume: 0.5–2.5 L

10 L Suspension Cell Culture Bag (without pH/DO sensors or retention membrane)

CS-CBSN-010

Without pH and DO sensors; Total Volume: 10 L; Working Volume: 1–5 L

20 L Suspension Cell Culture Bag (without pH/DO sensors or retention membrane)

CS-CBSN-020

Without pH and DO sensors; Total Volume: 20 L; Working Volume: 2–10 L

50 L Suspension Cell Culture Bag (without pH/DO sensors or retention membrane)

CS-CBSN-050

Without pH and DO sensors; Total Volume: 50 L; Working Volume: 5–25 L


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