Simplifying PBMC Cryopreservation: Direct Freezing of Whole Blood Application 2

Traditional PBMC workflows are complex: Whole blood → Ficoll separation → washing → resuspension → freezing

• Time-consuming, labor-intensive, and prone to cell loss or activation.

• CS-PM-D1 goes further: fully validated for direct whole blood freezing.

  ✔️ Mix whole blood 1:1 with CellStore PBMC cryomedium

  ✔️ Small volumes: direct storage at −80 °C

  ✔️ Large volumes: controlled-rate freezing

  ✔️ Post-thaw: no centrifugation needed, stain directly for flow cytometry

• Validated by data

  After thawing, lymphocyte subsets (T, B, NK cells) and surface marker expression closely match results from freshly analyzed whole blood—across multiple volumes and conditions.

  
  

  
  

• Fewer steps. Higher efficiency. More reproducible flow cytometry.

  
  

  
  

Sometimes, simpler really is better.