Homemade freezing medium or commercial freezing medium? After hearing this researcher's real experience, you'll probably know which one to pick. Anyone working with cell lines knows that heart-stopping moment during thawing.
Don't panic — here are two true “cell line cryopreservation MVPs”:
Both formulations are built specifically for cell line applications — and real-world data speaks louder than marketing.
“Our lab routinely cryopreserves cell lines. We froze the same batches with our homemade freezing medium and with your products. Same operators, same LN₂ tank, same maintenance schedule. For thawing, different colleagues handled each group for fairness. During the first month, the difference was barely visible— we even thought homemade medium was more cost-effective.
But after one year, the gap became huge:20% of the cells frozen with homemade medium didn’t recover, and several sensitive lines couldn’t be rescued at all. Your products had only ~5% failure rate, and the recovered cells were remarkably stable.
That’s when I realized — even with standardized workflows, homemade freezing medium
simply can’t withstand long-term cryostorage.”
✅ Optimized for major cell line categories Engineered (CHO, HEK293), cancer (786-O, 5637, A549, HUH7), and lymphocyte lines (Jurkat, E6-1, H9).
✅ High viability & strong post-thaw expansion Fast recovery, robust proliferation, and consistent performance in long-term culture.
✅ More stable growth behavior Healthy morphology, stable attachment/ suspension behavior, and improved experimental reproducibility.
✅ Truly ready-to-use No weighing, mixing, filtration, or last-minute prep — just freeze and go.
Stop letting homemade freezing medium slow down your experiments. These two formulations support different application needs and help your cells recover reliably and consistently — so your research can move faster.
