In PBMC cryopreservation, viability is often the first metric evaluated,But in real cell banking workflows, post-thaw quality also depends on whether the sample remains stable and manageable during downstream handling.
We recently reviewed post-thaw data from 3 customer PBMC samples cryopreserved with CS-PM-D1 in a PBMC cell bank-related setting, with storage periods ranging from approximately 3 to 6.5 months.
Across these samples, no visible flocculent formation was observed either immediately after thaw or after 30 minutes at 2–8°C. After dilution, centrifugation, and resuspension with different washing buffers, the suspensions also remained visually stable.
Another practical observation was that using culture medium as the washing buffer provided more consistent viability and recovery than saline-based buffers.
At CellStore, we believe post-thaw performance should be evaluated from a workflow perspective, not only by a single viability number. That is also why we continue to develop our cryopreservation media around ice growth control, with the goal of supporting both cell survival and more stable handling after thaw.
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