Class I medical device filing
GMP-compliant management with fully PRC Pharmacopoeia ingredients
Ready-to-use and long-term cryopreservation
High-viability and stable population
| Appearance | Colorless,pale yellow or yellow transparent liquid |
| Sterility | Sterile |
| Endotoxin Level | <0.5EU/mL |
| pH | 7.2±0.5 |
Collect the mononuclear cells from peripheral blood or cord blood,centrifuge the cell (500×g,5minutes,RT) and remove the supernatant.
Resuspend cell pellet with proper solution (saline,culture medium or DPBS) ,followed by cell counting and cell survival rate detection.A viability of over 90% before cryopreservation is preferred.Then centrifuge (500×g,5 minutes,RT) and remove the supernatant.
Resuspend cell pellet using the product to reach a cell density of 1×10⁶-1×108/mL and a freezing volume of 0.5-1.5 mL/tube.If using a cryopreservation bagforlarge volume cryopreservation,please contact the technical team to obtain the programed cooling curve.Users can also add autologous plasma,serum,or HSA according to experimental needs to improve post-thaw viability.
Transfer the cryovial to a-80℃ freezer.If long-term freezing is required,transfer to liquid nitrogen after 12hours.
1. After removing from liquid nitrogen,immediately immerse the cryovial into a 37℃ water bath,stir to rapidly thaw the cells within 2 minutes.
2. Transfer thawed cells to a conical tube,slowly add 9 times volume of proper diluent.
3. Equilibrate by gently invert the tube for 10 times.
4. Centrifuge (500×g,5 minutes,RT) and remove the supernatant.
5. Resuspend cell pellet with proper media/solution.
6. Perform cell count test if needed.
7. Proceed for downstream application.
Add thawed cells to 4 times the volume of complete culture medium,transfer to a centrifuge tube,and centrifuge for 5 minutes.Discard the supernatant,resuspend the cells using DPBS or complete culture medium,and then test the cell survival rate.
PBMC Cryopreservation Medium | 10% DMSO | 100ml | CS-PM-D1 |
For details about the products, delivery terms or price quote, please contact us!
The effect is negligible, as red blood cells need specific cryoprotectants and are susceptible to lysis and death at low temperatures.
Yes, data are available, including instances where cryopreserved PBMCs were thawed and cultured into T cells or NK cells using a PBMC cell culture protocol. These results can be explored in the provided external data documentation.
Tested densities include:
70 million (10^7)
1×10^8/mL
2×10^8/mL in PBMC freezing media
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