NK Cell Cryopreservation Medium

No, but this can be arranged upon request.

Both NK1 and NK3 cryopreservation solutions contain approximately 10% DMSO.

Not yet. All tests have been conducted in cryovials. We have evaluated NK cell stability in cryovials for up to 6 hours in cryopreservation solution before freezing and after thawing. For T cells, extensive cryobag testing has been performed.

NK cells naturally have low post-thaw viability, with clumping indicating dead cells. The NK cell source and medium are key factors. Optimizing the medium with human albumin helps. Cryopreservation solution (even CS10) has minimal impact. See our PPT for client data.

For freezing, use reverse pipetting (draw at second stop, dispense at first stop). Mix gently, avoid fully expelling the solution during pipetting, keep liquid in the tip, and release along the tube wall to avoid injecting directly into the suspension.

Yes, our brochures and PPT include post-thaw culture data at 12, 24, 36, and 48 hours.

Internal tests show viability at 2E8 and 5E8, but we recommend ≤2E8 for clients. Some clients successfully use 2E8.

Data are available for up to 12 months.

Yes.

Not yet. Current DMSO-free prototypes achieve >90% recovery but lack sufficient tumor cytotoxicity.